2nd Workshop on Malaria Molecular Surveillance – PDNA GEME
From October 21 to 26, 2024, the Project (PDNA GEME) organized an intensive training workshop on molecular surveillance of malaria for laboratory technicians of the PDNA’s countries National Malaria Control Programs (NMCPs). This workshop, led by Dr. Mamadou Tékété and Dr. Hinda Doucouré at the PDNA premises in Sotuba, aimed to equip participants with the skills needed to strengthen genomic malaria surveillance in Africa. The training was conducted under the supervision of Professor Abdoulaye Djimdé, President of the PDNA.
Participants and Objectives:
Participants from seven African countries including Cameroon, Gabon, Kenya, Mali, Madagascar, Niger, and Tanzania benefited from immersion in advanced molecular techniques for malaria detection and surveillance. The main objectives of this training were to:
- Strengthen the knowledge and skills of technicians in molecular biology applied to malaria.
- Train on diagnostic tools for genetic resistance of the Plasmodium falciparum parasite.
- Improve the interpretation and analysis of results for optimal management of malaria cases.
Content and Course of Training
The training week covered a wide range of laboratory techniques and practices, with the following highlights:
· Introduction to Molecular Surveillance Tools: Overview of new technologies applied to malaria surveillance.
- Sample Collection: Technique for realizing dried blood spot specimens (confetti), allowing optimized conservation and analysis.
- DNA Extraction: Methods for extracting DNA from dried blood spot.
- PCR (Polymerase Chain Reaction): Introduction to the principles and applications of PCR to detect molecular markers of resistance to antimalarials drugs.
- Detection of Resistance Markers: Use of PCR to identify the pfcrt and pfmdr1 genes mutations, responsible for resistance to certain antimalarial treatments.
- Identification of Plasmodium Species: Techniques for detecting different Plasmodium species, essential for surveillance and diagnosis.
- Interpretation of Results: Training on the analysis and interpretation of PCR results in order to make clinical decisions and inform public health actors.
Testimonial
The training was particularly enriching for the participants. According to Gemandze Ntsah Kelly, a participant from Cameroon:
“I am happy to have participated in the workshop on “Molecular Aspects of Malaria” organized by PDNA. The workshop covered various topics including the life cycle of the malaria parasite, diagnostic and treatment options. I particularly enjoyed the practical session where we learned how to identify the Pfcrt and Pfmdr1 resistance genes of Plasmodium falciparum and how to perform molecular correction with the Ta99 microsatellite. I look forward to applying the knowledge and skills acquired during this interactive training to my current studies and future projects.”
Conclusion and Presentation of Certificates
At the end of this training, each participant received a certificate of recognition. The satisfaction of the participants symbolized the success of the workshop, and their commitment to the fight against malaria in Africa. This workshop represents a significant step forward for NMCPs and for the capacity of African countries to monitor and control malaria more effectively. This type of training demonstrates the commitment of the PDNA in strengthening local skills for a better fight against infectious diseases in Africa.
